Preparation of uricase.
نویسنده
چکیده
منابع مشابه
The end-products and intermediates of uric acid oxidation by uricase.
Since the early work on uricase carried out by Schittenhelm (l), Wiechowski (a), and Battelli and Stern (3), and t,he elucidation of the structure of uric acid by the work of Behrend and Roosen (4), Fischer (5), and Biltz (6), the chemical nature of the intermediates and end-products of uricase action has been uncertain. The first indications of the structure of a possible intermediate were off...
متن کاملImmobilized-enzyme nylon-tube reactor for routine determination of uric acid in serum.
We report here the preparation of an immobilized-enzyme nylon-tube reactor containing uricase (EC 1.7.3.3) and the assembly of a flow-through system (Technicon AutoAnalyzer II) for the routine determination of uric acid in serum. Results of these uric acid analyses by use of immobilized uricase, in conjunction with peroxidase (EC 1.11.1.7) and aminophenazone-dichlorophenol in solution, are comp...
متن کاملPhylogeny of urate oxidase producing bacteria: on the basis of gene sequences of 16S rRNA and uricase protein
Uricase or Urate oxidase (urate:oxygen oxidoreductase, EC 1.7.3.3), a peroxisomal enzyme which is found in many bacteria, catalyzes the oxidative opening of the purine ring of urate to yield allantoin, carbon dioxide, and hydrogen peroxide. In this study, the phylogeny of urate oxidase (uricase) producing bacteria was studied based on gene sequences of 16S rRNA and uricase protein. Repres...
متن کاملکلونینگ و بیان آنزیم درمانی اوریکاز آسپرژیلوس فلاووس در اشریشیاکلی
Background and Objective: Uricase (EC 1.7.3.3) is an enzyme which catalyses uric acid to H2O2 and alantoin and is utilized in the treatment of hyperuricemia or gout. The aim of this study was cloning of Aspergillus flavus uricase synthetic gene into pET-28(a) and its recombinant expression in E. coli Materials and Methods: The coding sequence of uricase retrieved from gene bank and once opti...
متن کاملInduction and de novo synthesis of uricase, a nitrogen-regulated enzyme in Neurospora crassa.
Two efficient procedures are presented for the purification of the purine catabolic enzyme uricase from Neurospora crassa. A specific antiserum for uricase was prepared and used to examine the regulation of uricase expression. Even when wild-type cells are growing under full nitrogen repression conditions, they possess a considerable basal level of uricase. Induction results in a severalfold in...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 54 3 شماره
صفحات -
تاریخ انتشار 1953